Western Blot Analysis of Brain Homogenates

RIPA fractions of brain homogenates were used for Western blot analyses as previously described.^{10 (link), 15 (link), 16 (link)} Briefly, samples were elec trophoresed on 10% Bis–Tris gels or 3 to 8% Tris–acetate gel (Bio-Rad, Richmond, CA), transferred onto nitrocellulose membranes (Bio-Rad), and then incubated overnight with the appropriate primary antibodies as indicated in the Table. After 3 washings with T-TBS (pH 7.4), membranes were incubated with IRDye 800CW-labeled secondary antibodies (LI-COR Bioscience, Lincoln, NE) at room temperature for 1 hour. Signals were developed with an Odyssey Infrared Imaging System (LI-COR Bioscience). β-Actin was used as internal loading control.