Single-cell RNA-seq library preparation
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Corresponding Organization : Yale Cancer Center
Protocol cited in 13 other protocols
Variable analysis
- Composition of buffers
- Sequence of primers used
- Protocol steps
- Quality of the amplified DNA
- Quality of the libraries
- Captured mRNA reverse-transcription using Maxima H Minus reverse transcriptase
- CDNA coated beads treated with Exonuclease I
- Amplification cycles (13-16 cycles)
- Purification using Ampure XP beads
- Nextera tagmentation and amplification reactions
- Sequencing on HiSeq 2500 sequencer with custom primers
- PhiX libraries used as spike-in controls
- PhiX libraries used as spike-in controls
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