Antidiabetic activity of ajwain oil was assessed in differentiated L6 myotubes using fluorescent-tagged 6-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino)-2-deoxyglucose (6-NBDG). L6 myotubes (10,000 cells/well) were seeded in 96-well plates and allowed to confluence around 80%. Then, the cells were differentiated using 2% FBS and different concentrations of the oil (0.25–4 µL/mL) was added. At the end of treatment, 10 µM of insulin was added to stimulate glucose uptake and incubated for 15min. A total of 20 µg/200mL of 6-NBDG was added and incubated for 10min at dark. Glucose uptake (in percentage) was measured using Multimode reader (PerkinElmer) with an excitation/emission filter of 466/540nm.[14 (link)]
Protocol detail
Ajwain Oil Enhances Glucose Uptake
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization : Saveetha University
Top 5 similar protocols
Variable analysis
independent variables
- Different concentrations of ajwain oil (0.25–4 µL/mL)
- Glucose uptake (measured in percentage)
- L6 myotubes (10,000 cells/well) seeded in 96-well plates
- Cells allowed to reach ~80% confluence
- Cells differentiated using 2% FBS
- Insulin (10 µM) added to stimulate glucose uptake and incubated for 15 min
- 6-NBDG (20 µg/200 mL) added and incubated for 10 min in the dark
- Glucose uptake measured using Multimode reader (PerkinElmer) with an excitation/emission filter of 466/540 nm
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!