Matrigel Tube Formation Assay

The Matrigel tube formation was performed and analyzed as previously described, with minor modifications [21 (link)-23 (link)]. Namely, cells were added onto μ-slides Angiogenesis (Ibidi) and incubated at 37°C in 5% CO₂ in medium containing 0.5% FBS. Endothelial cell tube formation was assessed with an inverted photomicroscope (Olympus IX71) and experiments were conducted in triplicates and repeated at least three times.

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Jenkins S.V., Alimohammadi M., Terry A.S., Griffin R.J., Tackett A.J., Leung J.W., Vang K.B., Byrum S.D, & Dings R.P. (2021). Dysbiotic stress increases the sensitivity of the tumor vasculature to radiotherapy and c-Met inhibitors.. Angiogenesis, 24(3), 597-611.

Publication 2021

μ-slides Angiogenesis

Angiogenesis Cells Endothelial cell Matrigel

Corresponding Organization : University of Arkansas for Medical Sciences

Other organizations : University of Arkansas at Little Rock

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Endothelial cell tube formation

control variables

Incubation at 37°C in 5% CO2
Medium containing 0.5% FBS

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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