Protein from skin tissues and cultured cells were extracted for Western blotting as described (Ai et al., 2007 (link)). Primary antibodies were rabbit anti-mouse KLF4 (1:1000, GenSpin,), rabbit anti-mouse CXCR4 (1:1000, eBioscience) and rat anti-mouse β-actin (1:1000, Sigma-Aldrich).
Protocol detail
Protein Extraction and Western Blotting
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Corresponding Organization :
Other organizations : University of South Carolina, Southern Medical University, Union Hospital, Huazhong University of Science and Technology, University of Kentucky, University of Münster
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Variable analysis
independent variables
- Protein extraction from skin tissues and cultured cells
- Protein expression of KLF4 and CXCR4
- β-actin expression
- Positive control: Not specified
- Negative control: Not specified
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