Protein Expression Analysis Protocols

Western blot analysis was performed with whole cell lysates as described previously [8 (link)]. Membranes were incubated in enhanced chemiluminiscence solution (Thermo Scientific, Germany) and developed with the ImageQuant LAS500 system (GE Healthcare, Germany). Immunofluorescence staining was done as described elsewhere [7 (link)], and pictures were taken with the Fluorescence Microscope BX50F, Olympus XC30 Camera and cellSens Entry imaging software (Olympus, Germany). Antibodies and dilutions that were used for Western blot analysis and immunofluorescence staining are listed in Additional file 2.

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Grünow J., Rong C., Hischmann J., Zaoui K., Flechtenmacher C., Weber K.J., Plinkert P, & Hess J. (2017). Regulation of submaxillary gland androgen-regulated protein 3A via estrogen receptor 2 in radioresistant head and neck squamous cell carcinoma cells. Journal of Experimental & Clinical Cancer Research : CR, 36, 25.

Publication 2017

enhanced chemiluminiscence solution ImageQuant LAS500 system Olympus XC30 Camera

Antibodies Cell Dilutions Fluorescence microscope Immunofluorescence Membranes Western blot analysis

Corresponding Organization :

Other organizations : Heidelberg University, University Hospital Heidelberg, German Cancer Research Center

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Protein expression levels (as measured by Western blot analysis)
Protein localization (as measured by immunofluorescence staining)

control variables

None explicitly mentioned

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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