Samples were fixed and embedded as above and staining was performed
by hand using the Novus PD-L1 antibody NBP1–43262 at a 1:20 dilution.
The secondary antibody was from Vector Laboratories, BA-9401 goat anti-rat
at a 1:50 dilution. NIH3T3 cell pellet sections (mouse cells) were used as
positive controls, while mouse cSCC and mouse skin were exposed to secondary
antibody only as negative controls.
Mouse epidermal staining for PD-L1 was quantified using the ImagePro
Plus (Media Cybernetics software system, a Leica DMR microscope, and a Sony
3CCD color video camera [11 (link)]. The
mean positive cytoplasmic staining area per 20x field was determined for
each sample, measuring 3 fields/slide, 3 mice per time point.
by hand using the Novus PD-L1 antibody NBP1–43262 at a 1:20 dilution.
The secondary antibody was from Vector Laboratories, BA-9401 goat anti-rat
at a 1:50 dilution. NIH3T3 cell pellet sections (mouse cells) were used as
positive controls, while mouse cSCC and mouse skin were exposed to secondary
antibody only as negative controls.
Mouse epidermal staining for PD-L1 was quantified using the ImagePro
Plus (Media Cybernetics software system, a Leica DMR microscope, and a Sony
3CCD color video camera [11 (link)]. The
mean positive cytoplasmic staining area per 20x field was determined for
each sample, measuring 3 fields/slide, 3 mice per time point.
