RBCs and CBC2s were counted in C57BL/6J (hereafter B6/J) and A/J mice, and in 26 genetically distinct recombinant inbred (RI) strains derived from them comprising the AXB/BXA strain set. The cell count data from these mice have previously been reported in an on-line appendix (Keeley et al., 2014b (link)), while a more extensive study has recently reported the cell counts and quantitative trait locus (QTL) analysis for the RBC population (Kautzman et al., 2018 (link)); the methodology associated with these analyses is briefly recapitulated below. Eyes from Xkr8 knockout mice (KO) and heterozygous (Het) littermate control mice (wild type littermate controls were not available) were provided by the laboratory of Dr. Shigekazu Nagata at Osaka University in Japan (Suzuki et al., 2013 (link)). Electroporation experiments were conducted on CD1 mice originally obtained from Charles River Laboratories (Crl:CD1, #022), and subsequently bred in the UCSB Animal Resource Center. B6/J and A/J mice were also bred in-house, and used for in situ hybridization, immunofluorescence and qPCR studies. All experiments were carried out under authorization by the Institutional Animal Care and Use Committee at UCSB, and in accord with the NIH Guide for the Care and Use of Laboratory Animals.
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