Isolation and Culture of hMPCs

Skeletal muscle tissue and primary hMPCs were obtained using previously published methods (Riddle et al., 2018b (link)). Briefly, SkM tissue was taken from the vastus lateralis using the percutaneous biopsy technique. Tissue was snap-frozen in liquid nitrogen and stored at -80°C (SkM) or stored in Hibernate^®-A medium (Invitrogen) at 4°C until tissue disassociation. hMPCs were isolated as previously described (Xu et al., 2015 (link); Riddle et al., 2018b (link)) and were verified to be positive for the MPC specific transcription factor MyoD (Supplementary Figure S1). Passage six hMPCs were cultured in a 5% CO₂ atmosphere at 37°C on collagen (Type I, Rat Tail, Corning) coated plates. Only female hMPCs were used due to sample availability.