Cisplatin Resistance in NSCLC Cell Lines

Human non-small cell lung cancer (NSCLC) cell lines, A549 and H460 and monocytic cell line, THP-1 were purchased from the American Tissue Culture Collection (ATCC). Cells are maintained under the conditions recommended by the vendor. Cisplatin-resistant A549 and H460 cells were generated according to an established protocol [8 (link)] where a continued exposure to escalating concentration of cisplatin (CDDP) over the period of 6 months. Cisplatin [cis-diammineplatinum (II) dichloride] was purchased from Sigma-Aldrich and made into 0.20 M stock solution in NaCl and aliquoted. All cells were acquired or purchased with confirmations for the genotypic authentications from suppliers, and regularly tested for mycoplasma contamination.