Centipede DNA Extraction Protocol

Adult centipedes were collected from the field at a stretch of shingle bank beach near Brora, northeastern Scotland (coordinates: 57°59'N, 03°55'W) as described previously [31 (link)]. Once returned to the lab, individual adults were sexed according to morphological criteria [8 (link), 32 ]. The final leg-bearing segment and the genital segments were removed by dissection from female adults. This was to ensure complete removal of the spermatheca from female specimens, to avoid any contamination with male DNA. Adults were snap frozen in liquid nitrogen and stored at -80°C until processing. Genomic DNA (gDNA) was extracted from male and female specimens with the Genomic DNA Buffer Set and Genomic-tip 20/G (Qiagen) according to the manufacturer’s instructions.

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Green J.E., Dalíková M., Sahara K., Marec F, & Akam M. (2016). XX/XY System of Sex Determination in the Geophilomorph Centipede Strigamia maritima. PLoS ONE, 11(2), e0150292.

Publication 2016

Genomic DNA Buffer Set Genomic-tip 20/G

Adults Buffer Centipedes Dissection Dna contamination Female Frozen Genital Genomic Male Nitrogen

Corresponding Organization : Iwate University

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Variable analysis

independent variables

Specimen sex (male, female)

dependent variables

Genomic DNA (gDNA) extracted from male and female specimens

control variables

Liquid nitrogen storage temperature (-80°C)
Genomic DNA extraction protocol (Genomic DNA Buffer Set and Genomic-tip 20/G (Qiagen))

controls

Positive control: None mentioned
Negative control: None mentioned

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