Meningioma Cell Culture and Maintenance

Meningioma tumor samples were obtained intraoperatively from patients for whom tumor-banking consent were obtained. All experiments were performed in accordance with our institutional Research Ethics Board at the University Health Network (Toronto, Canada) and the University of Toronto. Cell suspensions were created and maintained as previously reported on ThermoFisher BioLite 100 mm Tissue Culture dishes in DMEM/F12 supplemented with 2 × non-essential amino acids (NEAA), 10 μg/mL gentamicin (Sigma-Aldrich) and fetal bovine serum (10% v/v; Life Technologies, Carlsbad, CA, USA) in an incubator at 37 °C and 5% CO₂. Once confluent, cells were passaged following trypsinization [28 (link), 30 (link)]. The following primary meningioma cell lines were used for in vitro drug screening: mng_20, mng_50, mng_84, mng_46. CH157 (CH-157MN; RRID:CVCL_5723) and IOMM-Lee (RRID:CVCL_5779) immortalized meningioma cell lines were obtained from the American Type Culture Collection (ATCC) and were cultured as monolayers in the same media composition as above.

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Wang J.Z., Patil V., Liu J., Dogan H., Tabatabai G., Yefet L.S., Behling F., Hoffman E., Bunda S., Yakubov R., Kaloti R., Brandner S., Gao A., Cohen-Gadol A., Barnholtz-Sloan J., Skardelly M., Tatagiba M., Raleigh D.R., Sahm F., Boutros P.C., Aldape K., Nassiri F, & Zadeh G. (2023). Increased mRNA expression of CDKN2A is a transcriptomic marker of clinically aggressive meningiomas. Acta Neuropathologica, 146(1), 145-162.

Publication 2023

gentamicin fetal bovine serum IOMM-Lee

Biolite Cell lines Cells Dishes Essential amino acids Fetal bovine serum Gentamicin Meningioma Patients Tissue Tumor

Corresponding Organization :

Other organizations : University of Toronto, Princess Margaret Cancer Centre, University Health Network, University Hospital Heidelberg, Heidelberg University, Bernstein Center for Computational Neuroscience Tübingen, University of Tübingen, National Hospital for Neurology and Neurosurgery, University College London, Indiana University Bloomington, National Institutes of Health, National Cancer Institute, Hertie Institute for Clinical Brain Research, Neurological Surgery, University of California, San Francisco, University of California, Los Angeles

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

None explicitly mentioned

control variables

Cell culture conditions (DMEM/F12 supplemented with 2 × non-essential amino acids, 10 μg/mL gentamicin, and 10% fetal bovine serum)
Incubation conditions (37 °C, 5% CO2)
Cell lines used (mng_20, mng_50, mng_84, mng_46, CH157, IOMM-Lee)

positive controls

None mentioned

negative controls

None mentioned

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