ChIP assays were performed according to protocols previously described (31 (link),33 (link)). In brief, cells were cross-linked with 1% formaldehyde for 10 min and then quenched with 0.125M glycine for 10 min. Cell pellets were resuspended in SDS Lysis buffer (1% SDS, 10 mM EDTA, 50 mM Tris-HCl, pH 8.0 and proteinase inhibitor cocktail) and sonicated for 30 min at 30 s intervals using a Bioruptor (Diagenode). A total of 4 µg of pre-cleared chromatin with Dynabeads protein A (Invitrogen) was immunoprecipitated using anti-OCT4, anti-H3K4me1, anti-H3K4me3 (Abcam), anti-H3K4me2, anti-H3K27me3, anti-H3K9/K14ac, anti-H4ac, anti-CTCF, anti-rabbit IgG (Millipore), anti-SOX2, anti-STAT3 (Cell Signaling Technology) or anti-SRF (Santa Cruz) antibodies. Quantification of the immunoprecipitated DNA was performed by qPCR and data are calculated as relative to the IgG-negative control and represented as enrichment of immunoprecipitated DNA samples corrected against the input DNA.