Glutinous rice husk from rice milling was obtained from Chiang Mai Phon Suriya Rice Mill, Co., Ltd., Chiang Mai, Thailand. The plant sample was identified and authenticated as O. sativa L. var. Niaw San-Pah-Tawng, of which the voucher specimen was kept under number 0023305 at the herbarium, Department of Pharmaceutical Science, Faculty of Pharmacy, Chiang Mai University.
The glutinous rice husk was ground into powder and extracted by aqueous enzymatic extraction using 0.5% v/v cellulase enzyme aqueous solution adjusted to pH 6.5 at a temperature of 50 °C for 24 h following the method of Jiamphun and Chaiyana (2022) [8 (link)]. After removing the glutinous rice husk residues by vacuum filtration through Whatman No. 1 filter paper using a Buchner funnel, the extracting liquid was removed by a freeze-dryer (FreeZone 4.5 model 7750031, Labconco, Kansas, MO, USA). The glutinous rice husk extract (CE0.5), with a yield of 1.9 ± 0.1% w/w [8 (link)], was kept in a well-closed container until the further experiments.
The glutinous rice husk was ground into powder and extracted by aqueous enzymatic extraction using 0.5% v/v cellulase enzyme aqueous solution adjusted to pH 6.5 at a temperature of 50 °C for 24 h following the method of Jiamphun and Chaiyana (2022) [8 (link)]. After removing the glutinous rice husk residues by vacuum filtration through Whatman No. 1 filter paper using a Buchner funnel, the extracting liquid was removed by a freeze-dryer (FreeZone 4.5 model 7750031, Labconco, Kansas, MO, USA). The glutinous rice husk extract (CE0.5), with a yield of 1.9 ± 0.1% w/w [8 (link)], was kept in a well-closed container until the further experiments.
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