The truncated form of Sec61b(45-97) was generated according to 37 (link)
, as the authors found that this Sec61b fragment decorated the ER without affecting microtubule bundling. GFP-Sec61β(133-291) was obtained by sub-cloning the C-terminus DNA fragment (133-291) (corresponding to aa 45 to 97) from the pAc-GFPC1-Sec61β (Plasmid #15108 Addgene) into the peGFP-C1 vector, using the following primers: Fwd (EcoRI) 5’- ATgaattctGGCCGCACAACCTCG-3’ and Rev (ApaI) 5’- ATTTgggcccCTACGAACGAGTGTACTTGCC-3’. This cloning was performed by the Aumeier Lab (University of Geneva, Switzerland).
, as the authors found that this Sec61b fragment decorated the ER without affecting microtubule bundling. GFP-Sec61β(133-291) was obtained by sub-cloning the C-terminus DNA fragment (133-291) (corresponding to aa 45 to 97) from the pAc-GFPC1-Sec61β (Plasmid #15108 Addgene) into the peGFP-C1 vector, using the following primers: Fwd (EcoRI) 5’- ATgaattctGGCCGCACAACCTCG-3’ and Rev (ApaI) 5’- ATTTgggcccCTACGAACGAGTGTACTTGCC-3’. This cloning was performed by the Aumeier Lab (University of Geneva, Switzerland).
