Measuring Oxidative Stress in Mycelia

DCFH-DA fluorescence staining was used to detect the level of ROS in mycelia. The treated mycelia were incubated in the dark for 30 min with DCFH-DA (final concentration, 10 μM). After washing with PBS buffer, the mycelia were observed using laser confocal microscopy (488 nm [excitation wavelength] and 525 nm [emission wavelength]) to measure ROS levels (72 (link)). Assay kits (BC3595, BC0175, BC0205, and BC0025; Solarbio Science & Technology Co., Ltd.) were utilized to measure H₂O₂ content, SOD activity, and MDA content, respectively, in treated mycelia.

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Zhang X.F., Li Q.Y., Wang M., Ma S.Q., Zheng Y.F., Li Y.Q., Zhao D.L, & Zhang C.S. (2022). 2E,4E-Decadienoic Acid, a Novel Anti-Oomycete Agent from Coculture of Bacillus subtilis and Trichoderma asperellum. Microbiology Spectrum, 10(4), e01542-22.

Publication 2022

BC3595 BC0175 BC0205 BC0025

Assay Buffer Dcfh da Fluorescence H2o2 Laser microscopy Mycelia

Corresponding Organization : Chinese Academy of Agricultural Sciences

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Variable analysis

independent variables

Treatment of mycelia

dependent variables

ROS levels
H2O2 content
SOD activity
MDA content

control variables

Incubation time of mycelia with DCFH-DA (30 min)
DCFH-DA concentration (10 μM)
Washing mycelia with PBS buffer

controls

No positive or negative controls were explicitly mentioned in the input.

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