A measure of 1 µg total RNA was used to synthesize cDNA using the Prime Script™ RT Reagent Kit (Takara, Kyoto, Japan). The qRT-PCR was performed with the SYBR Premix Ex Taq polymerase (Takara) according to the manufacturer’s instructions. The relative expression of selected DEGs were detected by CFX Connect Real-Time System (Bio-Rad Laboratories, Hercules, CA, USA). Pear PbrGAPDH were used as the internal controls [50 (link)]. The 2−ΔΔCT method was used to analyze the results [50 (link)]. Three biological replicates were performed for each sample. Primers used for RT-qPCR are listed in Supplementary Table S2 .
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