Protein Extraction and Western Blot Analysis

Protein extraction and WB assays were performed as previously described [28 (link)]. Primary antibodies targeting the following proteins were used in our WB analysis: Lamp2 (Proteintech, Wuhan, China, 27823-1-AP, 1:2000), EEA1 (Proteintech, 28347-1-AP, 1:2000), LC3B (Proteintech, 18725-1-AP, 1:2000), CD63 (Abcam, Burlingame, CA, USA, ab134045, 1:2000), GAPDH (Proteintech, 60004-1-Ig, 1:2000), NOS3 (Proteintech, 27120-1-AP, 1:2000), VAMP3 (Proteintech, 10702-1-AP, 1:2000), TSG101 (Proteintech, 28283-1-AP, 1:2000), Calnexin (Proteintech, 10427-2-AP, 1:2000).

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Hu Y., Zang W., Feng Y., Mao Q., Chen J., Zhu Y, & Xue W. (2024). mir-605-3p prevents liver premetastatic niche formation by inhibiting angiogenesis via decreasing exosomal nos3 release in gastric cancer. Cancer Cell International, 24, 184.

Publication 2024

Lamp2 GAPDH VAMP3 TSG101 Calnexin

Corresponding Organization : Macau University of Science and Technology

Other organizations : Nantong University, Affiliated Hospital of Nantong University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Protein expression levels of Lamp2, EEA1, LC3B, CD63, GAPDH, NOS3, VAMP3, TSG101, and Calnexin

control variables

None explicitly mentioned

controls

Positive controls: None mentioned
Negative controls: None mentioned

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