The production of superoxide was measured in the 15–20 mg fresh samples of the tissues using lucigenin (50 µmol/L)-enhanced chemiluminescence using a TriCarb 2910TR liquid scintillation analyser (TriCarb, Perkin Elmer, Waltham, MA, USA), as described previously by Kluknavsky et al. [26 (link)]. The results are expressed in the form of cpm/mg of wet tissue.
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