Quantification of Gene Expression by qRT-PCR

The expression levels of genes were quantified by qRT-PCR using gene-specific primers (Table S1) with ChamQ Universal SYBR qPCR Master Mix (Vazyme Biotech Co., Ltd. Nanjing, China) on a CFX Connect^TM Real-Time System (Bio-Rad, Hercules, California, America). A PCR reaction mix contained 10 μL of ChamQ Universal SYBR qPCR Master Mix (Vazyme Biotech Co., Ltd. Nanjing, China), 125 nM of each forward and reverse primer, 2.0 μL of cDNA template, and 7.5 μL of RNase-free water. Data analysis was calculated by the 2^−ΔCt method or 2^−ΔCtΔCt method [36 (link)]. All experiments were measured in three biological replicates.

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Liu G., Meng X., Ren Y., Zhang M., Chen Z., Zhang Z., Pang X, & Zhang X. (2022). Genes, Structural, and Biochemical Characterization of Four Chlorophyllases from Solanum lycopersicum. International Journal of Molecular Sciences, 23(19), 11716.

Publication 2022

ChamQ Universal SYBR qPCR Master Mix CFX ConnectTM Real-Time System

Biological Cdna Expression genes Gene Primer Rnase 5 Rnase 7

Corresponding Organization : South China Agricultural University

Other organizations : Fujian Agriculture and Forestry University

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Variable analysis

independent variables

Gene-specific primers

dependent variables

Expression levels of genes

control variables

ChamQ Universal SYBR qPCR Master Mix
CFX Connect™ Real-Time System
Reaction mix components (10 μL of ChamQ Universal SYBR qPCR Master Mix, 125 nM of each forward and reverse primer, 2.0 μL of cDNA template, and 7.5 μL of RNase-free water)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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