Hydroxocobinamide was synthesized from pharmaceutical grade hydroxocobalamin by base hydrolysis. The cobinamide product was >96% pure as determined using high-pressure liquid chromatography. It was converted to histidylcobinamide by adding four molar equivalents of histidine (Sigma-Aldrich) which was the compound administered in these experiments [11 (link)].
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Synthesis of Histidylcobinamide from Hydroxocobalamin
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Cobinamide
High pressure liquid chromatography
Histidine
Hydrolysis
Hydroxocobalamin
Molar
Pharmaceutical
Corresponding Organization : Health and Hospital Corporation
Other organizations : San Antonio Military Medical Center, Beckman Laser Institute and Medical Clinic, University of California, Irvine, United States Army Institute of Surgical Research, Pennsylvania State University, University of California, San Diego, Wilford Hall Medical Center
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Variable analysis
independent variables
- Adding four molar equivalents of histidine (Sigma-Aldrich) to the cobinamide product
- Purity of the histidylcobinamide compound
- Pharmaceutical grade hydroxocobalamin used as the starting material
- Base hydrolysis used to synthesize the cobinamide product
- High-pressure liquid chromatography used to determine the purity of the cobinamide product
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