The entire bowel movement was collected within 4 h of defecation and was transferred to the laboratory on ice under anaerobic conditions (Oxoid AnaeroGen Sachet; ThermoFisher Scientific)[9 (link)]. Samples were homogenised and aliquots were stored in – 80 °C. Faecal calprotectin was measured using the CALP0170 kit (CalproLab, Lysaker, Norway) [17 (link)].
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