WB analysis was performed as previously described [18 (link)]. First, the lysed cell supernatant was run on SDS-PAGE and then blotted with antibodies. Antibodies used in WB were as follows: anti-GP73 (Abcam, Cambridge, UK), anti-P65 (Cell Signaling Technology, Boston, MA, USA), anti-phosphorylated P65 (ImmunoWay Biotechnology Company, Plano, TX, USA), anti-IκBα, anti-phosphorylated IκBα, anti-IKKα/β (Santa Cruz Biotechnology, CA, USA), anti-GAPDH, and anti-β-actin (Abgent, San Diego, CA, USA).
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