In Vitro T Cell Stimulation Assays

For in vitro T cell stimulation assays, PMA (Sigma-Aldrich) was used at 50 ng/ml and ionomycin (Sigma-Aldrich) was used at 1 μg/ml final concentrations. For CsA (Calbiochem) treatment, 10x stock solutions were prepared in EtOH. CsA stocks were diluted with olive oil (Sigma-Aldrich) and, after thorough mixing, were injected i.p. at a dose of 80 mg/kg. For tacrolimus (Calbiochem), the final treatment dose was 2.5 mg/kg.

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Seth A., Yokokura Y., Choi J.Y., Shyer J.A., Vidyarthi A, & Craft J. (2023). AP-1–independent NFAT signaling maintains follicular T cell function in infection and autoimmunity. The Journal of Experimental Medicine, 220(5), e20211110.

Publication 2023

PMA ionomycin olive oil

Assays Etoh Ionomycin Olive oil T cell Tacrolimus

Corresponding Organization : Yale University

Top 5 similar protocols

Variable analysis

independent variables

PMA (Sigma-Aldrich) concentration
Ionomycin (Sigma-Aldrich) concentration
CsA (Calbiochem) treatment dose
Tacrolimus (Calbiochem) treatment dose

dependent variables

Not explicitly mentioned

control variables

EtOH for CsA stock preparation
Olive oil for CsA dilution

controls

Positive control: Not specified
Negative control: Not specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!