Three-Dimensional Bone Formation Analysis

To determine bone formation three-dimensionally, femurs were scanned in a SkyScan 1172 high-resolution µCT (Bruker, Kontich, Belgium). Voxel size was set to 8 µm and the bones were scanned with a source energy of 70 kV, 142 µA, a rotation step of 0.2 degrees and an 0.5 mm aluminum filter. Scans were reconstructed using NRecon (Bruker, Kontich, Belgium), applying ring artefact reduction and beam hardening corrections. CT Analyser software (version 1.20.3.0; both Bruker, Kontich, Belgium) was used for 2D and 3D analyses. By excluding the original cortical bone within the callus, the total volume (TV, mm³), the total bone volume (BV, mm³) and the bone volume fraction (BV/TV) of the newly formed bone were analyzed in a manually defined volume of interest (VOI)^{21 (link)}.

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Wolter A., Bucher C.H., Kurmies S., Schreiner V., Konietschke F., Hohlbaum K., Klopfleisch R., Löhning M., Thöne-Reineke C., Buttgereit F., Huwyler J., Jirkof P., Rapp A.E, & Lang A. (2023). A buprenorphine depot formulation provides effective sustained post-surgical analgesia for 72 h in mouse femoral fracture models. Scientific Reports, 13, 3824.

Publication 2023

SkyScan 1172 NRecon CT Analyser software

Aluminum Beam Bone Bone callus Cortical Cortical bone Femurs Formed bone Scans

Corresponding Organization : Freie Universität Berlin

Other organizations : Berlin Institute of Health at Charité - Universitätsmedizin Berlin, University of Basel, German Rheumatism Research Centre, University of Zurich

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Variable analysis

independent variables

Scanning parameters: voxel size (8 μm), source energy (70 kV, 142 μA), rotation step (0.2 degrees), aluminum filter (0.5 mm)

dependent variables

Total volume (TV, mm^3) of newly formed bone
Total bone volume (BV, mm^3) of newly formed bone
Bone volume fraction (BV/TV) of newly formed bone

control variables

Femurs scanned using a SkyScan 1172 high-resolution μCT
Reconstruction of scans using NRecon software, applying ring artifact reduction and beam hardening corrections
Analysis using CT Analyser software (version 1.20.3.0)

controls

No positive or negative controls were explicitly mentioned in the provided information.

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