Subcellular Localization of Soybean HMGR

GmHMGR4 and GmHMGR6 cDNA sequences were cloned into a pC1300s vector with the CaMV 35S promoter and eGFP (enhanced green fluorescent protein). The pC1300S-35S:GmHMGR4-GFP and pC1300S-E.R-mCherry-HDEL (plant endoplasmic reticulum markers) were transfected into A. thaliana mesophyll protoplasts, pC1300S-35S:GmHMGR6-GFP and pC1300S-E.R-mCherry-HDEL also go through the same transfection. On the second day following transfection, confocal laser scanning microscopy (OLYMPUS FV 1200) was used to detect the fluorescence from fusion proteins and organelle markers.

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Wang S., Feng Y., Lou Y., Niu J., Yin C., Zhao J., Du W, & Yue A. (2023). 3-Hydroxy-3-methylglutaryl coenzyme A reductase genes from Glycine max regulate plant growth and isoprenoid biosynthesis. Scientific Reports, 13, 3902.

Publication 2023

FV 1200

Cdna Confocal laser scanning microscopy Endoplasmic reticulum Enhanced green fluorescent protein Fluorescence Organelle Plant Proteins Protoplasts Transfection Vector

Corresponding Organization :

Other organizations : Shanxi Agricultural University

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Variable analysis

independent variables

Transfection of pC1300S-35S:GmHMGR4-GFP and pC1300S-E.R-mCherry-HDEL into A. thaliana mesophyll protoplasts
Transfection of pC1300S-35S:GmHMGR6-GFP and pC1300S-E.R-mCherry-HDEL into A. thaliana mesophyll protoplasts

dependent variables

Fluorescence detection from fusion proteins and organelle markers

control variables

Use of CaMV 35S promoter
Use of eGFP (enhanced green fluorescent protein)
Use of pC1300S-E.R-mCherry-HDEL (plant endoplasmic reticulum markers)
Confocal laser scanning microscopy (OLYMPUS FV 1200) used to detect fluorescence

controls

Positive control: pC1300S-E.R-mCherry-HDEL (plant endoplasmic reticulum markers)
Negative control: Not explicitly mentioned

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