Double Immunofluorescence Staining of B-Cells
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Corresponding Organization : United States Department of Veterans Affairs
Other organizations : University of Maryland, Baltimore, Memorial University of Newfoundland, Institute on Aging, National Institute on Aging, National Institutes of Health
Variable analysis
- Immunofluorescence staining protocol
- Localization of NS3 and core proteins in B-cells
- Incubation time (15 min)
- Temperature (room temperature)
- Blocking agent (3% BSA)
- Primary antibodies (anti-NS3, anti-core, anti-CD19, anti-CD20, anti-B220)
- Secondary antibodies (goat anti-rabbit Alexa Fluor 488, goat anti-mouse or anti-rat Alexa Fluor 597)
- Mounting medium (Vectashield)
- Positive control: None mentioned
- Negative control: Slides processed without primary antibodies
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