Multiparametric Flow Cytometry for PBMC Analysis

Distinct PBMC populations were enumerated by flow cytometry, as described (33 (link), 50 (link)). Phenotypic analyses were performed using Abs directed against CD3 (clone SP34-2), CD4 (L200), CD8 (RPA-T8), CD14 (M5E2), CD20 (2H2), CD45RA (5H9), CD56 (MY31) and CD127 (HIL-7R-M21) from BD Pharmingen (Franklin Lakes, NJ). Anti-CD159a (NKG2A) (Z199) was from Beckman Coulter and anti-CD28 (CD28.2) from Biolegend (San Diego, CA). Anti-CD25 (BC96) was from eBioscience (San Diego, CA) and anti-CD62L (SK11) from Becton Dickinson (Franklin Lakes, NJ). Data were acquired on a LSR II or LSR Fortessa (BD Bioscience) and analyzed with FlowJo software.

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Ezzelarab M.B., Zhang H., Guo H., Lu L., Zahorchak A.F., Wiseman R.W., Nalesnik M.A., Bhama J.K., Cooper D.K, & Thomson A.W. (2016). Regulatory T Cell Infusion Can Enhance Memory T Cell and Alloantibody Responses in Lymphodepleted Nonhuman Primate Heart Allograft Recipients. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 16(7), 1999-2015.

Publication 2015

CD127 (HIL-7R-M21) Anti-CD25 (BC96) LSR Fortessa

Cd25 Cd62l Clone Flow cytometry Phenotypic Populations

Corresponding Organization : University of Pittsburgh

Other organizations : University of Wisconsin–Madison

Top 5 similar protocols

Variable analysis

independent variables

None explicitly mentioned

dependent variables

Distinct PBMC populations enumerated by flow cytometry

control variables

None explicitly mentioned

controls

Positive controls: Not specified
Negative controls: Not specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!