Optimized Extraction of BZYQD Herbal Formula

The BZYQD contained 20 g of Atractylodes macrocephala Koidz, 24 g of Radix Bupleuri, 12 g of Citrus reticulata, 20 g of Ziziphus jujuba Mill, 20 g of Angelicae Sinensis Radix, 30 g of Codonopsis pilosula (Franch.) Nannf., 30 g of Astragalus membranaceus (Fisch.) Bunge, 12 g of Cimicifugae rhizoma, 42 g of Zingiber officinale Roscoe, and 30 g of Radix et Rhizoma Glycyrrhizae. Single herbs in the Chinese herbal BZYQD formula were obtained from the Wuxi Affiliated Hospital of Nanjing University of Chinese Medicine (Wuxi, China). In brief, the ten crushed herbs were mixed and soaked in 2L water at room temperature for 2 h and extracted by decocting twice for 30 min to obtain aqueous extracts. Then, the mixture was then filtered and concentrated to 1.04 g crude drug//mL as BZYQD for the experiments and stored at − 20 °C before use.