To create an MD mouse model, broad-spectrum antibiotics (ampicillin, 1 g/L; neomycin sulfate, 1 g/L; metronidazole, 1 g/L; vancomycin, 0.5 g/L; Sigma, USA) were added to the drinking water for 3 weeks to eliminate the intestinal flora, as described previously.11 (link) During this time, weight and food intake were recorded. At 2 days after antibiotic water was discontinued, to create the MD+KLA group, K. pneumoniae was administered.
Protocol detail
Antibiotic-Induced Gut Microbiome Modification
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Ampicillin
Antibiotic
Antibiotics
Food intake
Intestinal flora
K pneumoniae
Metronidazole
Mouse
Neomycin sulfate
Vancomycin
Corresponding Organization : Chaohu Hospital of Anhui Medical University
Other organizations : Anhui Medical University, First Affiliated Hospital of Anhui Medical University
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Variable analysis
independent variables
- Broad-spectrum antibiotics (ampicillin, 1 g/L; neomycin sulfate, 1 g/L; metronidazole, 1 g/L; vancomycin, 0.5 g/L) added to drinking water for 3 weeks
- Administration of K. pneumoniae 2 days after discontinuing antibiotic water
- Weight
- Food intake
- Elimination of intestinal flora by broad-spectrum antibiotics
- Positive control: Not specified
- Negative control: Not specified
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