Surface electrocardiography (ECG) was performed on sedentary animals at 6 and 12 months of age as described previously [21 (link)]. Briefly, mice were placed on a warming pad in a supine position and maintained under anesthesia with 1.5% isoflurane in oxygen via nose cone. Three-lead ECG probes were inserted subcutaneously in which the positive, negative, and ground electrodes were placed in the left hindlimb, right forelimb, and left forelimb, respectively. The electrodes were linked to a biopotential amplifier (BIOPAC MEC100C) connected to a computer interfaced BIOPAC MP150 system (BIOPAC Systems Inc., Goleta, CA) to measure and record electrical signals. Traces for each animal were recorded continuously for 30 min. Heart rate measurements and R–R intervals were calculated using Acqknowledge software.
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Measuring Cardiac Function in Mice
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Anesthesia
Animal
Cone
Electrical
Electrocardiography
Forelimb
Heart rate
Hindlimb
Isoflurane
Mice
Nose
Oxygen
Corresponding Organization :
Other organizations : University of Maryland, Baltimore, University of Arizona, University of Michigan–Ann Arbor
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Variable analysis
independent variables
- Age: 6 months, 12 months
- Heart rate measurements
- R-R intervals
- Mice were placed on a warming pad in a supine position
- Mice were maintained under anesthesia with 1.5% isoflurane in oxygen via nose cone
- Three-lead ECG probes were inserted subcutaneously in which the positive, negative, and ground electrodes were placed in the left hindlimb, right forelimb, and left forelimb, respectively
- No positive or negative controls were explicitly mentioned in the protocol.
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