Targeted Detection of APP and Tau Markers

Chemicals, including ARV-825 and JQ1, were from Medchemexpress (Catalog #s: HY-16954 and HY-13030, respectively) and their stocks were in DMSO. The G12A antibody was used to detect APP full length and C-terminal fragments (APP-CTFs) (rabbit polyclonal, clone C7 targeting amino acid residues 732–751 of APP751, custom made by Thermo Fisher Scientific) and were previously reported (57 (link), 58 (link), 59 (link)). The sAPPβ antibody was purchased from IBL (Catalog #: 18975). The 6E10 antibody (BioLegend) is a monoclonal antibody (mAb) reactive to amino acid residues 1–16 of Aβ from the N-terminal sequence and can detect sAPPα in medium. The nicastrin (NCT) antibody (Catalog #: 5665), BACE1 antibody (Catalog #: 5606), and LC3 a/b antibody (Catalog #: 12741) were purchased from Cell Signaling. The BRD4 antibody was from BETHYL (Catalog #: A700-004-T). The β-actin antibody was from Sigma and used as a protein control. The antibody for pTau (S396) was from Cell Signaling (Catalog #: 9632). The HRP-conjugated secondary antibodies (anti-mouse and anti-rabbit) were from Pierce. The dilution factors for primary and secondary antibodies were 1: 1000 and 1: 10,000, respectively.