Arabidopsis thaliana ecotypes Columbia (Col-0) and C24 were used as WT plants. Testcross experiments were conducted in gcs1/gcs1 (5 (link), 6 (link)), hap2-1/+ (9 (link)), lre/lre (15 (link)–17 (link)), and mea/mea (22 (link), 23 (link)) and in WT plants. The RPS5Apro::tdTomato-LTI6b (11 (link)) and CycB1;2pro:CycB1;2::NLS-YFP (12 (link)) lines were used for POEM assays. Seeds were sterilized with 5% sodium hypochlorite containing 0.5% Triton X-100 and germinated on plates containing 0.5× Murashige and Skoog salts (pH 5.7) (Wako Pure Chemical), 2% sucrose, Gamborg’s B5 vitamin solution (Sigma), and 0.3% Gelrite (Wako Pure Chemical) in a growth chamber at 21.5°C under 24 hours of light after cold treatment (4°C) for 2 days. Ten-day-old seedlings were transferred to Metro-Mix 350 soil (Sun Gro) and grown at 21.5°C under 24 hours of light.
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Arabidopsis Ecotypes and Mutants in Fertilization
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Corresponding Organization : Japan Science and Technology Agency
Other organizations : Chubu University, Kihara Institute for Biological Research, Yokohama City University
Top 5 similar protocols
Variable analysis
independent variables
- Genetic backgrounds of Arabidopsis thaliana plants
- Gcs1/gcs1
- Hap2-1/+
- Lre/lre
- Mea/mea
- Outcome of testcross experiments
- Arabidopsis thaliana ecotypes Columbia (Col-0) and C24 as WT plants
- RPS5Apro::tdTomato-LTI6b and CycB1;2pro:CycB1;2::NLS-YFP lines for POEM assays
- Seed sterilization with 5% sodium hypochlorite containing 0.5% Triton X-100
- Germination on 0.5× Murashige and Skoog salts (pH 5.7), 2% sucrose, Gamborg's B5 vitamin solution, and 0.3% Gelrite
- Growth conditions: 21.5°C, 24 hours of light, after cold treatment (4°C) for 2 days
- Transfer of 10-day-old seedlings to Metro-Mix 350 soil
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