Experiments were carried out on nanofibrous membranes made of a PLGA copolymer (ratio 85:15, Purasorb® PLG 8531; Corbion, Amsterdam, the Netherlands) or made of PLA (Ingeo™ Biopolymer 4032D; NatureWorks, Minnetonka, MN, USA). The solution for the polymers was prepared and the electrospinning process carried out as presented in our previous work.9 (link) Both polymers were dissolved in chloroform. Solvents – dichloroethane and ethyl acetate – were added into a PLA solution to a final concentration of 7 wt% of PLA. The volume ratio of the chloroform, dichloroethane, and ethyl acetate solvents was 61:29:10. The solution of the two polymers was made electrically conductive with the use of tetraethylammonium bromide. This chemical was first dissolved in dimethylformamide to a concentration of 3 wt%. Then, 3 g of this solution was added to 100 g of the PLGA or PLA solution.
Nanospider needle-free electrospinning technology (Elmarco, Liberec, Czech Republic) was used for preparing the nanofibrous membranes. The process conditions were electrode distance 145–180 mm, voltage 50–60 kV, relative humidity 20%–30%, and room temperature. Fiber density, ie, the area weight of the prepared nanofibers, was 10.5–19.6 g/m2 for PLGA and 13–15 g/m2 for PLA. The thickness of the membranes was in the range of 47–97 μm for PLGA and 125–190 μm for PLA.9 (link)
Nanospider needle-free electrospinning technology (Elmarco, Liberec, Czech Republic) was used for preparing the nanofibrous membranes. The process conditions were electrode distance 145–180 mm, voltage 50–60 kV, relative humidity 20%–30%, and room temperature. Fiber density, ie, the area weight of the prepared nanofibers, was 10.5–19.6 g/m2 for PLGA and 13–15 g/m2 for PLA. The thickness of the membranes was in the range of 47–97 μm for PLGA and 125–190 μm for PLA.9 (link)
