Immunohistochemical Analysis of CFTR

Primary airway epithelia were fixed in 10% Neutral Buffered Formalin (Leica Biosystems), paraffin embedded, and sectioned vertically. CFTR IHC was performed using mouse anti-CF-TR monoclonal antibody (769, CFFT) as previously described (55 (link)).

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Cooney A.L., Abou Alaiwa M.H., Shah V.S., Bouzek D.C., Stroik M.R., Powers L.S., Gansemer N.D., Meyerholz D.K., Welsh M.J., Stoltz D.A., Sinn P.L, & McCray PB J.r. (2016). Lentiviral-mediated phenotypic correction of cystic fibrosis pigs. JCI Insight, 1(14), e88730.

Publication 2016

Neutral Buffered Formalin

Cftr Epithelia Formalin Monoclonal antibody Mouse Paraffin

Corresponding Organization : University of Iowa

Other organizations : Institute of Molecular Biology and Biophysics, Howard Hughes Medical Institute

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Variable analysis

independent variables

Primary airway epithelia

dependent variables

CFTR expression

control variables

Formalin fixation
Paraffin embedding
Vertical sectioning

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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