The effect of standardized methanolic Ficus deltoidea varieties on insulin secretion was evaluated using clonal pancreatic beta cell line. BRIN BD11 were seeded at 2.5 x 105 cells/ml in 12 well plate and incubated for 24 hours at 37°C. Next, the cells were washed thrice with buffer and preincubated with Krebs-Ringer bicarbonate buffer for 40 minutes and continued for 60 minutes of incubation with the extracts (10-1000 μg/ml) or glybenclamide (10-2000 μg/ml). Ultrasensitive Rat Insulin ELISA kit (Mercodia AB, Sweden) was used to determine insulin concentration [5 (link)].
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