Morpholino Injection for Gene Knockdown

RIP3 e2/i2-splice-blocking (5′-TTTTAGAAATCACCTTGGCATCCAG-3′) and PGAM5-translation-blocking morpholino (5′-AGCGCCCTCCGAAAAGACATGCTTC-3′) (Gene Tools) were diluted to 0.15 mM in injection solution (4 (link)). Approximately 2-4 nl was injected into the yolks of embryos at the one-to-two-cell stage.

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Roca F.J., Whitworth L.J., Prag H.A., Murphy M.P, & Ramakrishnan L. (2022). TNF induces pathogenic mitochondrial ROS in tuberculosis through reverse electron transport. Science (New York, N.Y.), 376(6600), eabh2841.

Publication 2022

RIP3 e2/i2-splice-blocking PGAM5-translation-blocking morpholino

Cell Embryos Gene Morpholino Pgam5 Rip3

Corresponding Organization : MRC Laboratory of Molecular Biology

Other organizations : MRC Mitochondrial Biology Unit

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Variable analysis

independent variables

RIP3 e2/i2-splice-blocking (5′-TTTTAGAAATCACCTTGGCATCCAG-3′)
PGAM5-translation-blocking morpholino (5′-AGCGCCCTCCGAAAAGACATGCTTC-3′)

dependent variables

Not explicitly mentioned

control variables

Injection solution (4)

controls

Not mentioned
Not mentioned

Annotations

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