Intranasal Administration of Modified Vaccinia Virus

Recombinant modified vaccinia virus Ankara (MVA) viruses have been described previously and were generated by standard methods.^{4 (link),5 (link),23 (link)} In brief, the virus was propagated in chicken embryo fibroblasts, concentrated and purified by ultracentrifugation through sucrose. To determine the infectious units (IU) for use, viruses were titrated in chicken embryo fibroblasts. Before virus administration, mice were deeply anesthetized with a mixture of Ketamine (Albrecht GmbH) and Xylazine (Bayer Vital GmbH). MVA was diluted to a final concentration of 3.3 × 10⁸ IU/ml in PBS (Biochrom GmbH), and 30 μl (10⁷ IU) was administered intranasally.

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Mzinza D.T., Fleige H., Laarmann K., Willenzon S., Ristenpart J., Spanier J., Sutter G., Kalinke U., Valentin-Weigand P, & Förster R. (2018). Application of light sheet microscopy for qualitative and quantitative analysis of bronchus-associated lymphoid tissue in mice. Cellular and Molecular Immunology, 15(10), 875-887.

Publication 2018

Xylazine PBS

Chicken Embryo Fibroblasts Infectious Ketamine Mice Sucrose Ultracentrifugation Vaccinia virus Virus Xylazine

Corresponding Organization :

Other organizations : Medizinische Hochschule Hannover, University of Veterinary Medicine Hannover, Foundation, Center for Experimental and Clinical Infection Research, Helmholtz Centre for Infection Research, Ludwig-Maximilians-Universität München

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Variable analysis

independent variables

Recombinant modified vaccinia virus Ankara (MVA) viruses

dependent variables

Infectious units (IU)

control variables

Mice were deeply anesthetized with a mixture of Ketamine and Xylazine
MVA was diluted to a final concentration of 3.3 × 10^8 IU/ml in PBS
30 μl (10^7 IU) of MVA was administered intranasally

controls

No positive or negative controls were explicitly mentioned in the provided information.

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