Trastuzumab, T-DM1, and Paclitaxel Cytotoxicity

Cells were seeded into 24 well plates at a density of 0.1 x 10⁶ cells/ml and incubated alone or in 10 μg/ml trastuzumab (Herceptin, Roche Ltd., Basel, Swiss), 1 μg/ml T-DM1 (Kadcyla, Roche Ltd., Basel, Swiss), or 1μg/ml paclitaxel (Phyxol, Sinphar Ltd., I-Lan, Taiwan) for 72 hr [37 (link)]. At the end of this period, 0.1 ml of 0.4% trypan blue and deionized water (1:1) was added to the control and treated tubes to estimate the number of dead cells. Cell viability was estimated with a hemocytometer. Dead cells were stained blue, while live cells remained unstained. The cell mortality was expressed as the percentage of trypan blue-positive cells compared to the total number of cells. The viability percentage was presented by the number of live cells divided by the number of untreated control cells ×100.