Serological Analysis of Malaria Antigens

Samples were assayed for anti-malarial IgG antibody responses to P. falciparum and P. vivax merozoite surface protein (MSP-1₁₉) and apical membrane antigen 1 (AMA-1) recombinant protein [11 (link)] by enzyme-linked immunosorbent assay (ELISA) at the Amhara Region Health Research Laboratory Centre. Three-millimetre diameter circles were punched from DBS and reconstituted in PBS buffer as previously described [10 (link), 17 (link)]. Plates were coated with antigens at a concentration of 0.5 μg/mL overnight. Eluates from test samples were added at a dilution of 1:1000 and species-specific positive controls sera were added at serial dilutions on each plate to generate standard curves. After overnight incubation, horseradish-peroxidase-conjugated rabbit-anti-human IgG (DAKO, USA) secondary antibody was added followed by substrate Tetramethylbenzidine (TMB) (SurModics, USA) as color detection. Optical density (OD) values were read at 450 nm after 15 min incubation with the substrate.

Free full text: Click here

Yalew W.G., Pal S., Bansil P., Dabbs R., Tetteh K., Guinovart C., Kalnoky M., Serda B.A., Tesfay B.H., Beyene B.B., Seneviratne C., Littrell M., Yokobe L., Noland G.S., Domingo G.J., Getachew A., Drakeley C, & Steketee R.W. (2017). Current and cumulative malaria infections in a setting embarking on elimination: Amhara, Ethiopia. Malaria Journal, 16, 242.

Publication 2017

horseradish-peroxidase-conjugated rabbit-anti-human IgG

Anti igg Antibody Antibody responses Antigens Buffer Dilutions Enzyme linked immunosorbent assay Horseradish peroxidase Human Malarial Membrane Merozoite P protein Rabbit Recombinant protein Sera Tetramethylbenzidine Values

Corresponding Organization :

Other organizations : Amhara Regional Health Bureau, Program for Appropriate Technology in Health, University of London, Carter Center

Top 5 similar protocols

Variable analysis

independent variables

Antigens (P. falciparum and P. vivax merozoite surface protein (MSP-1₁₉) and apical membrane antigen 1 (AMA-1) recombinant protein)

dependent variables

Anti-malarial IgG antibody responses

control variables

Coating concentration of antigens (0.5 μg/mL)
Dilution of test samples (1:1000)
Dilution of positive control sera (serial dilutions)

controls

Positive controls: species-specific positive control sera
Negative controls: Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!