Serum protein concentration was determined by the method of Lowry. 16 Alkaline Copper sulfate solution catalyzes the oxidation of aromatic amino acids followed by the reduction of phosphomolybdic and phosphotungstic acid, resulting in a purple color complex whose intensity is proportional to the quantity of aromatic amino acid, which is measured at 660 nm.
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Lowry Protein Quantification Assay
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Other organizations : SASTRA University
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Variable analysis
independent variables
- Serum protein concentration
- Intensity of the purple color complex measured at 660 nm
- Alkaline Copper sulfate solution
- Positive control: Alkaline Copper sulfate solution catalyzes the oxidation of aromatic amino acids followed by the reduction of phosphomolybdic and phosphotungstic acid, resulting in a purple color complex.
- Negative control: Not explicitly mentioned.
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