At the end of the chronic pulmonary infection, animals were euthanized, and lungs harvested. Lungs were fixed overnight in 10% neutral buffered formalin (Merck, Gibbstown, NJ), then transferred to ethanol, embedded in paraffin, sectioned, and stained with H&E by the Mouse Tumor Model Resources (MTMR) at Roswell Park Comprehensive Cancer Center. Images were taken on an Olympus light microscope equipped with a CCD camera and Spot image analysis software (v25.4; Diagnostics Instruments, Sterling Heights, MI). Lung pathology was evaluated by a pathologist (P.N.B.) who was blinded to treatment groups, as described previously (10 (link)).
Protocol detail
Histological Lung Analysis in Chronic Infection
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Animals
Cancer
Chronic infection
Diagnostics
Embedded paraffin
Ethanol
Formalin
Lung
Microscope light
Mouse
Pathologist
Tumor
Corresponding Organization :
Other organizations : Roswell Park Comprehensive Cancer Center, University of Rochester
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Variable analysis
independent variables
- Chronic pulmonary infection
- Lung pathology
- Overnight fixation in 10% neutral buffered formalin
- Transfer to ethanol
- Embedding in paraffin
- Sectioning
- H&E staining
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