Peanut Allergy Protein Extraction

Peanut flour was purchased from the Golden Peanut Company (Alpharetta, Ga), endotoxin was undetectable (<0.5 EU/mg flour) as previously described (7 (link)). Crude peanut extract (7 (link)) and Alternaria alternata extract (19 (link)) were purchased from Greer Laboratories (Lenoir, NC). Recombinant IL-1α was purchased from R&D Systems (Minneapolis, MN), and recombinant IL-13 was purchased from Biolegend (San Diego, Ca).

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Krempski J.W., Kobayashi T., Iijima K., McKenzie A.N, & Kita H. (2020). Group 2 Innate Lymphoid Cells Promote Development of T follicular Helper (Tfh) Cells and Initiate Allergic Sensitization to Peanuts. Journal of immunology (Baltimore, Md. : 1950), 204(12), 3086-3096.

Publication 2020

Crude peanut extract Alternaria alternata extract Recombinant IL-1α recombinant IL-13

Alternaria alternata Crude extract Endotoxin Flour Il 13 Peanut

Corresponding Organization :

Other organizations : WinnMed, Mayo Clinic, Mayo Clinic in Arizona, MRC Laboratory of Molecular Biology, Medical Research Council

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Variable analysis

independent variables

Peanut flour purchased from the Golden Peanut Company (Alpharetta, Ga)
Crude peanut extract (7 (link))
Alternaria alternata extract (19 (link))
Recombinant IL-1α purchased from R&D Systems (Minneapolis, MN)
Recombinant IL-13 purchased from Biolegend (San Diego, Ca)

dependent variables

Not explicitly mentioned

control variables

Endotoxin level was undetectable (<0.5 EU/mg flour) as previously described (7 (link))

controls

Positive control: Not mentioned
Negative control: Not mentioned

Annotations

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