Inducible Adiponectin Knockout Mouse Model

Animal care and experimental protocols were approved by the Institutional animal care and use committee of the University of Texas Southwestern medical center. Adiponectin-RTTA mice were produced as previously described (9 (link)). Tre-Cre (Stock Number: 006234) and PTEN floxed mice (Stock Number: 006440) were purchased from Jackson laboratories. All experiments were done using male mice with littermate controls that were adiponectinP-rtTA or Tre-Cre negative. Mice were fed a standard chow diet (number 5058, LabDiet), a Dox-chow diet (600 mg kg⁻¹ Dox; Bio-Serv), a 60% HFD (D12492, Research Diets Inc.) or a Dox-HFD (600 mg kg⁻¹ Dox; Bio-Serv). Following weaning mice were randomly placed into cages for the remainder of their life before genotyping was performed. No other randomization was used while conducting experiments. No animals were excluded from any experiments unless they displayed obvious wounds from fighting as determined by our veterinarians.