Protocol detail

Mouse Models of Soft Tissue Sarcoma

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All animal experiments were performed according to protocols approved by the Duke University Institutional Animal Care and Use Committee. Mouse models of soft tissue sarcoma were generated in a mixed 129/SVJae and C57BL/6 background using a combination of previously described alleles: Pax7^Cre-ER-T2 (provided by Chen-Ming Fan, Carnegie Institution for Science) [20 (link)], p53^FL (provided by Anton Berns, The Netherlands Cancer Institute) [44 (link)], Hif1a^FL (provided by Randall Johnson, University of Cambridge) [45 (link)], and Nras^LSL-G12D [46 (link)] and ROSA26^mTmG [47 (link)] which were obtained from The Jackson Laboratory. (Z)-4-hydroxytamoxifen (Sigma Aldrich), which is referred to as 4-OHT, was first dissolved in 100% ethanol at a concentration of 250mg/ml, and then diluted with corn oil (Sigma Aldrich) to a final working solution concentration of 25mg/ml. Primary mouse soft tissue sarcomas were generated in the mouse hind limb by intramuscular (IM) injection of 20μl 4-OHT working solution via a 27.5 gauge insulin syringe (Terumo).

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Zhang M., Qiu Q., Li Z., Sachdeva M., Min H., Cardona D.M., DeLaney T.F., Han T., Ma Y., Luo L., Ilkayeva O.R., Lui K., Nichols A.G., Newgard C.B., Kastan M.B., Rathmell J.C., Dewhirst M.W, & Kirsch D.G. (2015). HIF-1α regulates the response of primary sarcomas to radiation therapy through a cell autonomous mechanism. Radiation research, 183(6), 594-609.

Publication 2015

(Z)-4-hydroxytamoxifen corn oil

4 hydroxytamoxifen Alleles Cancer Corn oil Ethanol Hind limb Institutional animal care and use committee Insulin Intramuscular injection Mouse Soft tissue sarcomas Syringe

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Protocol cited in 2 other protocols

Variable analysis

independent variables

Pax7^Cre-ER-T2
P53^FL
Hif1a^FL
Nras^LSL-G12D
ROSA26^mTmG
(Z)-4-hydroxytamoxifen (4-OHT)

dependent variables

Generation of primary mouse soft tissue sarcomas in the mouse hind limb

control variables

Mouse background (mixed 129/SVJae and C57BL/6)
Injection volume (20μl)

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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