W3, TPC-1 and Nthy-ori 3–1 were purchased from Cell Bank of Chinese Academy of Sciences and were cultured in DMEM medium (Gibco, Carlsbad, CA) containing 10% FBS (Gibco) and 1% pen/strep (Gibco) in a humidified atmosphere at 37 °C with 5% CO2. HUCMSCs were isolated from fresh umbilical cord samples as previously described [17 (link), 18 (link)] and maintained in stem cell culture medium (Cyagen, Guangzhou, China).
Free full text: Click here