Profiling Inflammation Response in Lewisite-Exposed Skin

RNA (100 ng) from vehicle or lewisite-exposed skin samples was hybridized overnight at 65°C to the nanoString nCounter mouse Inflammation V2 panel (nanoString Technologies, Seattle, WA, USA) comprising 254 genes. Hybridized samples were then immobilized onto an nCounter cartridge and imaged on an nCounter SPRINT Profiler (nanoString Technologies) as previously described (25 (link)). Data were analyzed with nSolver Analysis Software (nanoString Technologies). The threshold for significant differential expression was |1| for log fold change and p<0.05 for t-test significance.

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Srivastava R.K., Mishra B., Muzaffar S., Gorbatyuk M.S., Agarwal A., Mukhtar M.S, & Athar M. (2022). Dynamic Regulation of the Nexus Between Stress Granules, Roquin, and Regnase-1 Underlies the Molecular Pathogenesis of Warfare Vesicants. Frontiers in Immunology, 12, 809365.

Publication 2021

nanoString nCounter mouse Inflammation V2 panel nCounter SPRINT Profiler nSolver Analysis Software

Genes Inflammation Lewisite Mouse Skin

Corresponding Organization : University of Alabama at Birmingham

Other organizations : Birmingham VA Medical Center

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Variable analysis

independent variables

Exposure to vehicle or lewisite

dependent variables

Gene expression levels of 254 genes in the nanoString nCounter mouse Inflammation V2 panel

control variables

RNA input amount (100 ng)
Hybridization temperature (65°C)
Hybridization duration (overnight)

controls

No positive controls specified
No negative controls specified

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