Quantitative RT-PCR Analysis of Gene Expression

A PrimeScript RT enzyme with a gDNA eraser (Takara, Japan) was used for cDNA synthesis. QPCR was performed on a Bio-Rad CFX96 Real Time PCR system using SYBR Premix ExTaq II (Takara, Japan). The primers in this step were listed in Supplementary Table S1. Tung Elongation Factor 1-α (EF1α) was used as the internal control (Han et al., 2012 (link)). The relative expression levels were calculated using the 2^-ΔΔCT method (Livak and Schmittgen, 2001 (link)). Phytohormone levels were analyzed using the methods described by Pan et al. (2002) .

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Liu M., Li W., Zhao G., Fan X., Long H., Fan Y., Shi M., Tan X, & Zhang L. (2019). New Insights of Salicylic Acid Into Stamen Abortion of Female Flowers in Tung Tree (Vernicia fordii). Frontiers in Genetics, 10, 316.

Publication 2019

PrimeScript RT enzyme with a gDNA eraser CFX96 Real Time PCR system SYBR Premix ExTaq II

Cdna Elongation factor 1 α Enzyme Phytohormone Primers Synthesis Tung

Corresponding Organization : Central South University

Other organizations : Henan Institute of Science and Technology

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Relative expression levels of genes
Phytohormone levels

control variables

Tung Elongation Factor 1-α (EF1α) as the internal control

controls

Positive control: None mentioned
Negative control: None mentioned

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