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RNA Isolation and qRT-PCR Analysis

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Total RNA was isolated using a modified Trizol extraction protocol49 (link). First-strand cDNA was synthesized using PrimScript™ RT reagent Kit with gDNA Eraser (Takara). Quantitative real-time RT-PCR was performed on ABI Prism 7900HT (Applied Biosystems) with the SYBR Green system. The gene specific primers used in qRT-PCR analysis were listed in Supplementary Table S6. The Actin gene was used as internal control.

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Liu C., Long J., Zhu K., Liu L., Yang W., Zhang H., Li L., Xu Q, & Deng X. (2016). Characterization of a Citrus R2R3-MYB Transcription Factor that Regulates the Flavonol and Hydroxycinnamic Acid Biosynthesis. Scientific Reports, 6, 25352.

Publication 2016

PrimScript™ RT reagent Kit with gDNA Eraser ABI Prism 7900HT

Actin Cdna Gene Primers Prism Quantitative real time pcr Sybr green Trizol

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Protocol cited in 1 other protocol

Variable analysis

independent variables

Modified Trizol extraction protocol
PrimScript™ RT reagent Kit with gDNA Eraser (Takara)
ABI Prism 7900HT (Applied Biosystems)
SYBR Green system
Gene specific primers

dependent variables

Quantitative real-time RT-PCR results

control variables

Actin gene as internal control

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