Cell cycle analysis was performed on untreated and 24 h treated cells with Rapha Myr® extract (0.5–1.25–2.5% v/v), according to Malfa et al., 2019 [70 (link)]. In order to assess cell cycle state, 1 × 106 cells were stained with propidium iodide (PI, 50 ng/mL) and analyzed by the FlowSight® (Amnis®, part of EMD Millipore) imaging flow cytometer. The cell distribution in each cell-cycle phase was determined by using IDEAS 6.0 image analysis software (Amnis Inc., Seattle, WA, USA).
Tomasello B., Di Mauro M.D., Malfa G.A., Acquaviva R., Sinatra F., Spampinato G., Laudani S., Villaggio G., Bielak-Zmijewska A., Grabowska W., Barbagallo I.A., Liuzzo M.T., Sbisà E., Forte M.G., Di Giacomo C., Bonucci M, & Renis M. (2020). Rapha Myr®, a Blend of Sulforaphane and Myrosinase, Exerts Antitumor and Anoikis-Sensitizing Effects on Human Astrocytoma Cells Modulating Sirtuins and DNA Methylation. International Journal of Molecular Sciences, 21(15), 5328.
Corresponding Organization : University of Catania
Other organizations :
Polish Academy of Sciences, Instytut Biologii Doświadczalnej im. Marcelego Nenckiego, Institute of Biomedical Technologies, National Research Council, Italian Association for Cancer Research
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